Lonomia obliqua venom and hemolymph have been shown to contain molecules that, besides interfering in blood coagulation (for a review, Veiga et al. ). Lonomia obliqua (Walker, ) is a moth from the family Saturniidae, widely distributed in tropical rainforests of South America. In its larval stage (caterpillar) it . Rev Assoc Med Bras (). May-Jun;61(3) doi: / Lonomia obliqua Walker (Lepidoptera: Saturniidae): hemostasis .
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Universidade Estadual Paulista, Instituto Butantan. The histology is poorly lonomua owing to the coagulopathy-preventing biopsies in the acute phase of the illness. The coagulopathy becomes apparent, typically within 48 h after poisoning, presenting with ecchymosis and the bleeding complications described [ 24 ].
Effect of the antiapoptotic protein on the viability of quiescent UMG cells. De Lima ME, editor. The antiapoptotic protein from the hemolymph of L.
Effects of Lonomia obliqua caterpillar venom upon the proliferation and viability of cell lines
Accordingly, our results show that the venom increases the viability of tumor cells and decreases the viability of fibroblasts, which may be due to molecular differences between cell types. Exploring new molecules and activities from Lonomia obliqua caterpillars. It is typical in that the systemic illness was preceded by an immediate local cutaneous reaction with burning pain, erythema and oedema. Even though some active principles seem to be concentrated in the spicules, there is no morphological barrier separating these structures from the hemolymph, therefore different venomous preparations may have similar activities Pinto et al.
Ana Beatriz Gorini da Veiga, Phone: All experiments were performed at least three times. Briefly, the caterpillars were placed in Petri dishes on ice. We express special thanks to Dr.
This obliaua also occurs in normal cells, in which proliferation is well regulated, which does not occur in tumor cells. Both results of this study, either an increase or a decrease in cell proliferation, are important.
Can J Physiol Pharmacol. Physical examination revealed several skin llonomia, and gross hematuria was present. It is known that NO has an important role in the progression of gliomas Himlin et al. Onliqua effects of a dose from multiple caterpillars can be dramatic and severe, including massive internal hemorrhagingrenal failureand hemolysis.
Two species of Lonomia, the Brazilian caterpillar Lonomia obliqua and the Venezuelan caterpillar Lonomia achelous, provoke activation of the onliqua cascade through the action of several compounds that are the subject of toxicology research [ 2 ]. Animal venoms are complex combinations of different active principles, causing diverse effects in human physiology when envenomation occurs.
[Accidents with caterpillar Lonomia obliqua (Walker, 1855). An emerging problem].
In order to investigate whether the observed effects of the venom on cell viability and proliferation are due to a direct activity or an indirect activity that might involve cell signaling through the cAMP pathway, experiments of cell viability and proliferation using the venom in combination with agents such as forskolin, which increases cAMP levels in the cell, and 8-Br-cAMP, a c-AMP analogue, were performed.
Hematoma and gangrene -like symptoms manifested, spreading throughout the body, eventually causing massive blood leakage into the brain and, in several cases, death.
Acute renal failure provoked by toxin from caterpillars of the species Lonomia obliqua. A typical envenomation incident involves a person unknowingly leaning against, placing their hand on, or rubbing their arm against a group of these caterpillars that are gathered on the trunk of a tree.
To increase cell yield, many procedures are used, such as supplementing the culture medium with nutrients and mitogenic activators, minimizing the presence of toxic metabolites such as lactate and ammonia, and preventing the lack of oxygen.
Caterpillars of many species can cause irritation by their hollow body hairs that envenom or detach easily, or can be poisonous if ingested;  however, prior to investigations into Lonomia caterpillars, it was not known that caterpillars could produce toxins which in sufficient quantities could kill a human being. A molecule with a potent activity in increasing cell proliferation and viability is of great interest in the biotechnology industry. While there are more than a dozen species in the genus, the most troublesome species is Lonomia obliquaand it is this species on which most of the medical research has centered.
Losac, a factor X activator from Lonomia obliqua bristle extract: When travelling to endemic zones in Central and South America, travellers should be aware of poisonous caterpillars and avoid direct contact. These mechanisms of increasing or decreasing cellular proliferation are extremely important for the understanding of the venom effects in different cell types and should be a lonmia subject for future studies.
Brazilian Journal of Medical and Biological Research. Venom samples were prepared from bristles as described in Veiga et al.
[Accidents with caterpillar Lonomia obliqua (Walker, ). An emerging problem].
Accidental poisoning with caterpillars has become increasingly frequent in southern Brazil, partly due ogliqua deforestation and elimination of natural predators [ 1 ]. Exponentially growing cells were detached with trypsin 0.
Acute renal failure and haemorrhagic syndrome secondary to toxin of caterpillars Lonomia obliqua Miguel C.