Egyptian Henbane learn more about names for this taxon Hyoscyamus muticus Trusted Image of Hyoscyamus muticus; Map of Hyoscyamus muticus. Expert(s). Expert: Notes: Reference for: Other Source(s). Source: Germplasm Resources Information Network (GRIN), – , database (version ). Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

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Genetic improvement of Egyptian henbane, Hyoscyamus muticus L.

In addition we also report reliable morphological markers to identify putative tetraploids in this species. This study showed that stomata characteristics markedly differed between diploid and tetraploid plants. The ploidy level of treated plants near their flowering stage, was measured by flow cytometry Partec PA, Germany.

The plant survival was not affected by the concentration and time of exposure of colchicines. Survival and growth of colchicine treated nuticus The initiation of growth occurred about 10 muicus after treatment, whereas untreated plantlets continue to hyoscyamsu without any delay.

The stem histograms showed no significant difference with those of leaves, while the root histogram showed a higher difference. It has been reported that the tetraploid plant, Hyoscyamus muticushad nearly 1. Plant Cell Tissue Organ Cult.

The most widely applied and best studied chemical that induces polyploids is colchicine, an alkaloid extracted from seeds or corms of the autumn crocus Colchicum autumnae or Colemid, its synthetic equivalent Hassawi and Liang, ; Poehlman, The first visible effect of colchicines was the delayed growth of treated plantlets.

The Tuareg people use it as a fish poison.

Hyoscyamus muticus – Wikipedia

The results of this study also indicate that colchicine treatment can efficiently be used to improve Hyoscyamus muticus for commercial and pharmaceutical applications of its vegetative organs. Morphological characteristics such as size and thickness of leaves, flowers, seeds, epidermis and guard cells, as well as the number of chloroplast in guard cells were considered as other parameters for identifying putative tetraploid plants.


Although, all of the plantlets were affected by the colchicines, small number of plantlets showed some browning and burning at the place of treatment. A common model for explaining changes, originating from tetraploid induction, relies on the assumption that the lower ratio of nuclear membrane to chromatin the volume of tetraploid cells is typically about double and their surface area is about 1.

Hyoscyamus muticus

However, this agent works effectively only on dividing cells, thus polyploidization generally hyoscyaums not equally occur in all explant cells, leading to the occurrence of mixoploids and chimeras Wan et al. For the stomata measurements of the above plantlets, the upper epidermis of leaves was removed and stuck on microscopic slides. The leaves may also be applied as a poultice to relieve pain.

Increased numbers of chloroplast in the stomata guard cells of tetraploid plants compared to diploid ones is another consequence of polyploidy induction Gu et al. In vitro induction of tetraploid plants from diploid Zizyphus jujuba Mill. Increased size of stomata guard cells is one of the general effects of tetraploidy induction in the Egyptian henbane, as shown in Fig. Afterwards pre-germinated seeds with newly emerged mutixus were transferred to small pots containing vermiculite-perlite-peat mixture.

The G 2 peak of tetraploids is approximately on channelwhile diploids showed a G 2 peak on channel When released slowly, scopolamine reduces the nausea of motion sickness or post-operative recovery. The meristem tip of plantlets in their leaf stage were first covered with cotton Fig.


Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Representative body size, flowers, seed sizes and leaves from diploid and colchiploid plants of Egyptian henbane Hyoscyamus muticus a, b body size, c, d relative size of flowers, e, f relative size of seeds and g, h leaves of diploid and tetraploid, respectively.

The characteristics of treated plantlets C 0 and their progeny C 1 were compared with non-treated ones control. Endopolyploidy in hyosvyamus and tetraploid maize Zea mays L. Polyploidy manipulation has been successfully used in plant breeding to facilitate the production of superior cultivars in many crop hyoscyqmus.

The fruit is a capsule. In most cases, the first leaves of tetraploids had a distored appearance, but the subsequent leaves appeared normal at the place of treatment. As for this day, no reports of a relationship between stomata chloroplast number and ploidy level in Egyptian henbane have been made.

Plants Profile for Hyoscyamus muticus (henbane)

Solid, stable autotetraploids as well as mixoploids were produced by treating Egyptian henbane plantlets with colchicine. The body size of diploids and tetraploids were significantly different, tetraploids being stronger than diploids one Fig. In other hyoscyamuw Wikimedia Commons Wikispecies.

In vitro induction of tetraploids in colchicine-treated cocoyam plantlets. In vitro induction of tetraploidy in mulberry Morus alba L. Pakistan Journal of Biological Sciences, It muticuz alkaloids that are useful in pharmaceuticals. How to cite this article: Mixoploid plants had a peak on channel with 8n nuclei that corresponds to the G 2 phase of tetraploid cell cycle Fig. T, Sapra and S.