API (Analytical Profile Index) 20E presented is a biochemical panel for identification and differentiation of members of the family. Changes to thesaurus. Changes to database old new. Taxons. Notes. Identification. Additional tests. API® 20 E v v X. X. X. X. RapiD 20 E™ v v X. API® 20 Microbial Identification Kits, bioMerieux. Supplier: Quantity. API® 20 E Microbial Identification Kit, , , , Pack of 25, Retrieving.
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The interpretation of mathematically calculated identification results requires trained clinical personnel who should use judgement and knowledge in conjunction with the following information before accepting the ID of an organism: The 12B strips can be used alongside the 12E, but in a separate tray. The lysine and ornithine decarboxylase reactions should be carefully interpreted.
For aid in interpreting reactions, refer gakerie the Table of Reactions. Bromothymol blue indicator changes from blue to yellow when the carbohydrate is utilised to form acid. Production of a red colour within a few minutes of the addition of the reagent indicates that nitrate reduction to nitrite NO 2 has occurred.
Twelve substrates provide insufficient data to speciate within this group galerue a single aberrant reaction may result in an incorrect identification. Utilisation of Na malonate at the same time that ammonium sulphate is utilised as the nitrogen source produces sodium hydroxide resulting in increased alkalinity and a blue colouration.
General Aspects and Perspectives. In addition, motility and nitrate test should be performed for miscellaneous Gram-negative bacilli. The results of tests for oxidase, nitrate reduction and motility are included as part of the reaction pattern.
API PANEL READING
The 24E solid microplate format contains the 24 substrates contained in the combination of both the 12A and 12B strips. Kit presentation Each kit contains the following: H 2 S is produced from thiosulphate.
To determine the purity of the inoculum, it is advisable to inoculate a solid non-selective medium with the test suspension to act as a culture purity check. Using the results obtained, the indices of the positive reactions are circled.
Incubation Reseal the inoculated rows with the adhesive seal and write the specimen identification number on the end tag with a marker pen. All systems should be read after 48 hours for the galerke of Miscellaneous Gram-negative bacilli.
Solid gelatin particles which may drift across the well after rehydration should be considered as a negative reaction. Each group of three reactions produces a single digit of the code. Thomas, Veterinary Microbiology. This will determine whether nitrate has been reduced completely to nitrogen gas N2.
MBA Microbact 12E 20 pouches, identifications. These can be purchased individually or as a set by ordering product code MB This causes a pH rise as indicated by bromothymol blue. Set up proceedure Isolation An hour pure culture of the organism to be identified must be obtained. The 12A strip may be used alone for identification of oxidase-negative, nitrate-positive glucose fermenters comprising 15 genera and may be useful for screening pathogenic Enterobacteriaceae from enteric and urine specimens or identification of other common isolates.
The 12B strip can be used in conjunction with the 12A strip for the identification of oxidase-positive, nitrate-negative, and glucose-nonfermenters MGNB as well as the Enterobacteriaceae. Preparation of inoculum Pick isolated colonies from an hour culture and emulsify in 2. The reactions are evaluated as positive or negative by comparing them with the colour chart. When Actinobacillus or Pasteurella spp.
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Gram-stain, colonial morphology, source of isolate, percent probability degree of separationtests against, additional test indications and results, frequency of ID choice and antibioGram. The pH shift indicated by bromothymol blue caused by formation of the specific amine putrescine is greater than that caused by lysine decarboxylation.
All organisms belonging to the family Enterobacteriaceae reduce nitrates to nitrites and give a positive reaction. The following organisms are recommended for independent laboratory assessment.
Record the results under the appropriate heading on the report form.