Download Citation on ResearchGate | Actinobacillus seminis isolated from cattle | The organism commonly referred to as Actinobacillus. Actinobacillus seminis is a well known cause of epididymitis in Australian rams ( Baynes and Simmons, ) and has also been reported as associated with. The main causative agents of ovine infectious epididymitis are Brucella ovis, Actinobacillus seminis, and Histophilus somni [1–3].
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Nonparametric data testicular consistency and length and width of the tail of epididymes were analyzed by Kruskal Wallis, followed by Dunn test for multiple comparisons. Baynes I, Simmons G. Proteins were electrotransferred 18 to 0.
Immunoblot analysis of Actinobacillus seminis microvesicles, showing recognition of the kDa protein by antibody against A. This makes prevention and management of A. Comparison and partial characterization of the protein profiles and outer membrane antigens of Actinobacillus species isolated from ram lambs with epididymitis.
To visualize the A. This article has been cited by other articles in PMC. View actnobacillus Google Scholar G. Conversely, as these infections are very similar, the importance of complementary diagnostic techniques is evident for confirmation of the etiologic agent in cases of ovine infectious epididymitis.
These results suggest that the kDa protein could be specific to A.
PCR methods for rapid identification and characterization of Actinobacillus seminis strains. This disease has been reported in all major sheep producing regions in the world, including Brazil. The analyses were performed with the GraphPad Actinobadillus program, version 3. Support Center Support Center. InSeminix et al 10 reported having separated proteins from strains of A.
Contagious ovine epididymitis is defined as infection of the testicle and epididymis in rams. The isolate was further investigated by API32E biochemical testing which didn’t result in a confirmed identification. B, extract from A. It is noteworthy that, in cases of chronic epididymitis, testicular consistency can increase irreversibly as a result of atrophy and interstitial fibrosis [ 824 ]. This is an open access article distributed under the Creative Commons Attribution Licensewhich permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Polyclonal serum anti- A. An invertebrate coagulation system activated by endotoxin: Al-Katib and Dennis recommended continual surveillance against the introduction of infected rams into a clean flock and identifying and removing infected animals.
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The bacterial isolate was catalase positive and oxidase negative. Bacterial isolates were grouped according to protein profile. Ovine infectious epididymitis is one of the major causes of reproductive disorders in sheep, and it strongly impacts on the productivity of the herds [ 1 ]. Actinobacillus seminis is actinobcaillus gram-negative bacterium of the Pasteurellaceae family that is involved in ovine epididymitis.
B, Western-blot results, showing recognition of the kDa protein in A. To detect the kDa protein in membrane microvesicles of A. As Figure 2A shows, Western-blot tests of whole-cell extracts with rabbit hyperimmune serum against A.
Materials and methods Bacterial strains and growth conditions The bacterial species used in this study are shown in Table I.
Several previous studies evaluated the pathological changes in rams experimentally infected with B. There was no bacterial recovery from inguinal and iliac lymph nodes, spleen, and liver. B, corresponding results of Western blot testing with rabbit antibody against A. There is a actiobacillus report of A.
This protocol was repeated whenever necessary. To confirm the infection, semen, urine, and blood were obtained prior to inoculation and every seven dpi, during six weeks.
To determine which proteins of A. Among the etiologic agents of epididymitis are several bacterial species, such as Brucella ovis, Actinobacillus seminis, Mannheimia haemolytica Pasteurella haemolyticaPasteurella multocida, and Histophilus somni Haemophilus somnus 3.
The polypeptide with a molecular mass of 75 kDa identified in the fractions and had an apparently specific immunogenic reaction: Whole blood was collected by jugular vein puncture with vacuum collection system.
Group 1 consisted of A. Unfortunately this was not practical in this case. Open in a separate window. This technique is performed until the animal shows the characteristic signs of the infection. Nevertheless, the frequency of positive tissues was similar between both groups. Membrane vesicles released by Actinobacillus pleuropneumoniae contain proteases and Apx toxins.
To the best of our knowledge, this is the 1st report of data on the proteins in subcellular fractions of A.